Monospecific antibody against Bordetella pertussis Adenylate Cyclase protects from Pertussis

Objectives: Acellular pertussis vaccines has been largely accepted world-wide however, there are reports about limited antibody response against these vaccines suggesting that multiple antigens should be included in acellular vaccines to attain full protection. The aim of present study was to evaluate the role of Bordetella pertussis adenylate cyclase as a protective antigen. Materials and methods: Highly mono-specific antibody against adenylate cyclase (AC) was raised in rabbits using nitrocellulose bound adenylate cyclase and the specificity was assessed by immuoblotting. B.pertussis 18-323, was incubated with the mono-specific serum and without serum as a control. Mice were challenged intra-nasally and patho physiolgical responses were recorded. Results: The production of B.pertussis adenylate cyclase monospecific antibody that successfully recognized on im munoblot and gave protection against fatality (p< 0.01) and lung consolidation (p <0.01). Mouse weight gain showed significant difference (p< 0.05). Conclusion: These preliminary results highlight the role of the B.pertussis adenylate cyclase as a potential pertussis vaccine candidate. B.pertussis AC exhibited significant protection against pertussis in murine model. J Microbiol Infect Dis 2012; 2(2): 36-43