ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation | Zendy

Kristýna Šmerková | Zendy; Marcela Vlčnovská | Zendy; Simona Dostálová | Zendy; Vedran Milosavljević | Zendy; Pavel Kopel | Zendy; Tomáš Vaculovič | Zendy; Soňa Křížková | Zendy; Markéta Vaculovičová | Zendy; Vojtěch Adam | Zendy; René Kizek | Zendy

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ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation

Author(s) -

Kristýna Šmerková,

Marcela Vlčnovská,

Simona Dostálová,

Vedran Milosavljević,

Pavel Kopel,

Tomáš Vaculovič,

Soňa Křížková,

Markéta Vaculovičová,

Vojtěch Adam,

René Kizek

Publication year - 2015

Publication title -

nanobiomedicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.561

H-Index - 12

ISSN - 1849-5435

DOI - 10.5772/61699

Subject(s) - cisplatin , dna , guanine , adduct , fluorescence , chemistry , antibody , dna adduct , combinatorial chemistry , biophysics , microbiology and biotechnology , biochemistry , genetics , biology , nucleotide , organic chemistry , chemotherapy , gene , physics , quantum mechanics

Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized.

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