Hematopoietic Differentiation from Embryonic Stem Cells
Author(s) -
Yasuhisa Yokoyama,
Hidekazu Nishikii,
Shigeru Chib
Publication year - 2011
Publication title -
intech ebooks
Language(s) - English
Resource type - Book series
DOI - 10.5772/16086
Subject(s) - embryonic stem cell , haematopoiesis , microbiology and biotechnology , stem cell , biology , genetics , gene
Since the development of human embryonic stem cells (hESCs) in 1998, the potential of stem cell-based manufacturing of tissues or organs as a form of regenerative medicine has drawn broad interest because hESCs are pluripotent and can proliferate infinitely without losing their pluripotency (Thomson et al., 1998). More recently, induced pluripotent stem cells (iPSCs) were generated from human fibroblasts (Takahashi et al., 2007) as well as other cell sources (Stadtfeld and Hochedlinger, 2010), thus accelerating the goals of research to realize regenerative medicine. Theoretically, any organ can be generated from ESCs, but the obstacles to manufacturing solid organs in vitro remain great. Solid organs such as kidney and liver require wellfunctioning three-dimensional structures consisting of different kinds of cells as well as formation of and communication with blood vessels. Considering this, hematopoietic progenitors or mature blood cells derived from ESCs may be among the most attractive applications because blood cells can operate as single cells without forming a multicellular structure. Here, we describe methods of hematopoietic differentiation from ESCs, particularly focusing on hESCs, and some problems that need to be resolved before hESCderived blood cells can be applied in the clinical setting.
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