Open AccessAdult/Pediatric Nutritional Formula by Liquid Chromatography: First Action 2011.20
Author(s) -
Brendon D. Gill,
Harvey E. Indyk,
Maureen C. Kumar,
Nathan K. Sievwright,
Merilyn ManleyHarris,
Dawn Dowell
Publication year - 2012
Publication title -
journal of aoac international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 87
eISSN - 1944-7922
pISSN - 1060-3271
DOI - 10.5740/jaoacint.cs2011_20
Subject(s) - inosine , chromatography , guanosine , uridine , chemistry , repeatability , high performance liquid chromatography , cytidine , inosine monophosphate , adenosine , biochemistry , nucleotide , enzyme , rna , gene
A method for the routine determination of 5'-mononucleotides (uridine 5'-monophosphate, inosine 5'-monophosphate, adenosine 5'-monophosphate, guanosine 5'-monophosphate, and cytidine 5'-monophosphate) in infant formula and adult nutritionals is described. After sample dissolution and addition of internal standard, potential interferences were removed by anion-exchange SPE followed by HPLC-UV analysis. Single-laboratory validation performance parameters include recovery (92-101%) and repeatability (1.0-2.3% RSD). The method was approved for Official First Action status by an AOAC expert review panel.
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