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period. It is well known that fetal chromosomal trisomies increase with advanced maternal age [1]. In the 1980s, the risk of mid-trimester trisomy 21 in a 35-years-old woman at delivery was reported to be 1:270. This risk rapidly rises with increasing maternal age over 35 years. The maternal-age-related risk of Observed frequency of fetal trisomy between 16 and 24 gestational weeks in pregnant women older than 34 years at delivery