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Assessing REM Sleep in Mice Using Video Data
Author(s) -
Blakeley B. McShane,
Raymond J. Galante,
Michael P. Biber,
Shane T. Jensen,
Abraham J. Wyner,
Allan I Pack
Publication year - 2012
Publication title -
sleep
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.222
H-Index - 207
eISSN - 1550-9109
pISSN - 0161-8105
DOI - 10.5665/sleep.1712
Subject(s) - non rapid eye movement sleep , wakefulness , epoch (astronomy) , eye movement , electroencephalography , sleep stages , vigilance (psychology) , psychology , audiology , slow wave sleep , sleep (system call) , computer science , statistics , polysomnography , neuroscience , mathematics , medicine , computer vision , stars , operating system
Assessment of sleep and its substages in mice currently requires implantation of chronic electrodes for measurement of electroencephalogram (EEG) and electromyogram (EMG). This is not ideal for high-throughput screening. To address this deficiency, we present a novel method based on digital video analysis. This methodology extends previous approaches that estimate sleep and wakefulness without EEG/EMG in order to now discriminate rapid eye movement (REM) from non-REM (NREM) sleep.

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