Open AccessKINETICS OF QUERCETIN NITRATION BY HORSERADISH PEROXIDASE
Author(s) -
Andrija Šmelcerović,
Nataša Sokolov,
Živomir Petronijević
Publication year - 2013
Publication title -
acta medica medianae
Language(s) - English
Resource type - Journals
eISSN - 1821-2794
pISSN - 0365-4478
DOI - 10.5633/amm.2013.0201
Subject(s) - horseradish peroxidase , nitration , quercetin , chemistry , kinetics , biochemistry , organic chemistry , enzyme , physics , quantum mechanics , antioxidant
In this study we investigated the kinetics of the nitration of quercetin by horseradish peroxidase. Quercetin nitration reaction was followed by recording the spectral changes over the time at 380 nm. The reaction rate increases with increasing of the quercetin concentration and follows the Michaelis-Menten type kinetics. Kinetic parameters of the studied enzymatic reaction were determined
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom