Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease | Zendy

Dhony Hermanto | Zendy; Mudasir Mudasir | Zendy; Dwi Siswanta | Zendy; Bambang Kuswandi | Zendy; Nurul Ismillayli | Zendy

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Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease

Author(s) -

Dhony Hermanto,

Mudasir Mudasir,

Dwi Siswanta,

Bambang Kuswandi,

Nurul Ismillayli

Publication year - 2019

Publication title -

journal of mathematical and fundamental sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.216

H-Index - 12

eISSN - 2337-5760

pISSN - 2338-5510

DOI - 10.5614/j.math.fund.sci.2019.51.3.8

Subject(s) - chitosan , urease , membrane , polyelectrolyte , chemistry , chemical engineering , immobilized enzyme , nuclear chemistry , fourier transform infrared spectroscopy , calcium alginate , chromatography , enzyme , polymer , organic chemistry , biochemistry , calcium , engineering

PEC of the alginate-chitosan membrane as supporting material for immobilizing urease was produced. This study aimed to develop a supporting material for enzyme immobilization that has high stability, a fast response time and an easy and relatively inexpensive preparation procedure. An alginate-chitosan PEC membrane was produced by reacting alginate hydrosol and chitosan (1:1 in mass) at pH 5.28, followed by mixing and drying at room temperature. The FTIR spectra, XRD patterns and SEM assay confirmed that alginate-chitosan PEC was obtained. The color change of the BTB indicator proved that urease was trapped in the cavities of the alginate-chitosan PEC membrane while the immobilized urease still showed catalytic activity. Thus, the membrane of alginate-chitosan PEC has good characteristics as a matrix for urease immobilization.

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