Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis | Zendy

Elin Julianti | Zendy; Susanti Susanti | Zendy; Marlia Singgih Wibowo | Zendy; Laida Neti Mulyani | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis

Author(s) -

Elin Julianti,

Susanti Susanti,

Marlia Singgih Wibowo,

Laida Neti Mulyani

Publication year - 2019

Publication title -

journal of mathematical and fundamental sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.216

H-Index - 12

eISSN - 2337-5760

pISSN - 2338-5510

DOI - 10.5614/j.math.fund.sci.2019.51.2.6

Subject(s) - spirulina (dietary supplement) , phycocyanin , extraction (chemistry) , chromatography , pigment , chemistry , size exclusion chromatography , cyanobacteria , biology , biochemistry , bacteria , organic chemistry , raw material , genetics , enzyme

Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freeze-thawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research