An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices | Zendy

Angélica GodínezOviedo | Zendy; Gerardo M. Nava | Zendy; Sofía M. ArvizuMedrano | Zendy; Montserrat HernándezIturriaga | Zendy

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An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices

Author(s) -

Angélica GodínezOviedo,

Gerardo M. Nava,

Sofía M. ArvizuMedrano,

Montserrat HernándezIturriaga

Publication year - 2017

Publication title -

polish journal of microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.312

H-Index - 34

eISSN - 2544-4646

pISSN - 1733-1331

DOI - 10.5604/01.3001.0010.7869

Subject(s) - listeria monocytogenes , false positive paradox , biology , protocol (science) , polymerase chain reaction , listeria , microbiology and biotechnology , computational biology , gene , genetics , bacteria , medicine , computer science , artificial intelligence , alternative medicine , pathology

Several studies have observed that a conventional PCR protocol using primers LM1 and LM2 for the identification of gene hlyA Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason in this study, we provide a modified PCR protocol that improves the specificity of the LM1 and LM2 primers.

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