Open AccessCharacterization of Microbial Communities in Acidified, Sulfur Containing Soils
Author(s) -
Grzegorz Czerwonka,
Iwona Konieczna,
Paulina Żarnowiec,
Artur Zieliński,
Agnieszka Malinowska-Gniewosz,
Agnieszka Gałuszka,
Zdzisław M. Migaszewski,
Wiesław Kaca
Publication year - 2017
Publication title -
polish journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.312
H-Index - 34
eISSN - 2544-4646
pISSN - 1733-1331
DOI - 10.5604/01.3001.0010.7043
Subject(s) - sulfur , soil water , bioremediation , microorganism , bacteria , isolation (microbiology) , bacillus (shape) , 16s ribosomal rna , chemistry , soil microbiology , sulfate , environmental chemistry , biology , microbiology and biotechnology , ecology , genetics , organic chemistry
Over a period of three years, microbial communities in acidified soil with high sulfur content were analyzed. In soil water extracts ureolytic, proteolytic, oxidoreductive, and lipolytic activity were detected. The presented results indicate that the enzymatic activity of soil microbial communities varied considerably over time. Isolated 26 (80%) bacterial strains belonged to genus Bacillus sp. and were identified by cultivation and 16S rRNA methods. The commercially available procedures for bacterial DNA isolation from acidified soil failed, therefore a new, specific DNA isolation method was established. Ureolytic activity, detected in soil extracts as well as in isolated Bacillus sp. strains may be considered as a tool for the bioremediation of acidified soils with high sulfate content.
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