Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363 | Zendy

Pushpendra Awadhiya | Zendy; Tushar Banerjee | Zendy; Shridhar Patil | Zendy

Open Access

Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363

Author(s) -

Pushpendra Awadhiya,

Tushar Banerjee,

Shridhar Patil

Publication year - 2017

Publication title -

polish journal of microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.312

H-Index - 34

eISSN - 2544-4646

pISSN - 1733-1331

DOI - 10.5604/01.3001.0010.4858

Subject(s) - bioconversion , biotransformation , substrate (aquarium) , chemistry , solvent , chromatography , bioreactor , biochemistry , enzyme , nuclear chemistry , organic chemistry , biology , fermentation , ecology

Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, temperature, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml-1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.

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