Pilosella bauhinii (Schult.) Arv.-Touv. and P. cymosa subsp. vaillantii (Tausch) S. Bräut. & Greuter (Asteraceae) from new localities in north-central Poland

We provide information about two species, Pilosella bauhinii and P. cymosa subsp. vaillantii (Asteraceae), from new localities in north-central Poland (ATPOL DC18). All individuals of both species grew on a railway embankment in Górzno-Lidzbark Landscape Park. The ecological preferences for P. cymosa and P. bauhinii estimated according to average Ellenberg values nearly coincided with the original description of Ellenberg. We also sequenced the barcode marker, plastid trnH-psbA intergenic spacer, for two individuals of P. bauhinii, three specimens of P. cymosa subsp. vaillantii, and additionally two individuals of P. officinarum L. and one H. murorum L. growing in close proximity. A pairwise comparison of trnH-psbA sequences showed that each species has a unique haplotype. Taking into account their morphological coherence, it is possible that both P. bauhinii and P. cymosa have not hybridized yet, at least locally, with the more abundant species (e.g., P. officinarum). A search for reference sequences did not provide additional information because of the low quality of the reference database for this group in GenBank. Only 14 sequences of trnH-psbA were available with some apparently being misidentified or of low quality. None were identical to sequences of P. cymosa subsp. vaillantii and P. bauhinii found in this study