Isolation of bacteria from ectomycorrhizae of Tuber aestivum Vittad | Zendy

Milana Gryndler | Zendy; Hana Hršelová | Zendy

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Isolation of bacteria from ectomycorrhizae of Tuber aestivum Vittad

Author(s) -

Milana Gryndler,

Hana Hršelová

Publication year - 2013

Publication title -

acta mycologica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.38

H-Index - 5

eISSN - 2353-074X

pISSN - 0001-625X

DOI - 10.5586/am.2012.018

Subject(s) - ectomycorrhizae , biology , mycelium , bacteria , 16s ribosomal rna , isolation (microbiology) , rhizosphere , botany , hypha , horticulture , microbiology and biotechnology , mycorrhiza , symbiosis , genetics

Fifteen different cultivation media were used to isolate bacteria with the idea to obtain taxa specifically associated with ectomycorrhizae of Tuber aestivum. Ectomycorrhizae were collected at the sampling points previously analyzed for bacterial molecular diversity. We isolated 183 bacterial strains and identified them on the basis of the partial sequence of 16S rDNA. Out of these isolates, only 4 corresponded to operational taxonomic units significantly associated with T. aestivum ectomycorrhizae in previous molecular study. Preliminary study of the effect of 12 selected isolates on growth of T. aestivum mycelium showed no stimulation and one isolate induced the damage of hyphae. Different isolation strategy has to be developed to increase the probability of cultivation of potentially important components of T. aestivum mycorrhizosphere

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