Characterization and Prokaryotic expression of Glucuronyltransferase-S Gene in Silkworm Bombyx mori | Zendy

Yukun Chen | Zendy; Keping Chen | Zendy; Fang Bao | Zendy; Qin Yao | Zendy

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Characterization and Prokaryotic expression of Glucuronyltransferase-S Gene in Silkworm Bombyx mori

Author(s) -

Yukun Chen,

Keping Chen,

Fang Bao,

Qin Yao

Publication year - 2009

Publication title -

international journal of biology

Language(s) - English

Resource type - Journals

eISSN - 1916-968X

pISSN - 1916-9671

DOI - 10.5539/ijb.v1n1p104

Subject(s) - bombyx mori , genbank , gene , in silico , cloning (programming) , biology , complementary dna , genome , exon , genetics , isoelectric point , protein superfamily , amino acid , identification (biology) , biochemistry , enzyme , botany , computer science , programming language

As genome of B. mori is available in GenBank, identification of novel genes of B. mori can be carried out. In this study, we used the in silico cloning method to obtain the Glucuronyltransferase-S (GlcAT-S) gene of B. mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR,prokaryotic expression and western blot. The GlcAT-S cDNA contains a 843bp ORF and has three exons. The deduced protein has 280 amino acid residues, with the predicted molecular weight of 31842. 02 Da, isoelectric point of 9.16, and contains conserved GlcAT domains. The protein shows high degrees of identity with that of some homologous protein from other species

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