The Use of Fourier Infrared Spectroscopy and Laser – Raman Spectroscopy in Bladder Malignancy Diagnosis, A comparative Study | Zendy

Essam G. Ahmed | Zendy; Nafie A. Almuslet | Zendy; Mubarak M. Ahmed | Zendy; M. A. Moharam | Zendy; Waleed Musaad | Zendy

Open Access

The Use of Fourier Infrared Spectroscopy and Laser – Raman Spectroscopy in Bladder Malignancy Diagnosis, A comparative Study

Author(s) -

Essam G. Ahmed,

Nafie A. Almuslet,

Mubarak M. Ahmed,

M. A. Moharam,

Waleed Musaad

Publication year - 2010

Publication title -

applied physics research

Language(s) - English

Resource type - Journals

eISSN - 1916-9647

pISSN - 1916-9639

DOI - 10.5539/apr.v2n1p108

Subject(s) - raman spectroscopy , bladder cancer , fourier transform infrared spectroscopy , materials science , laser , spectroscopy , malignancy , analytical chemistry (journal) , infrared spectroscopy , infrared , bladder neoplasm , nuclear magnetic resonance , cancer , optics , medicine , pathology , chemistry , chromatography , physics , organic chemistry , quantum mechanics

Bladder cancer is one of the most common cancers in Africa. It takes several days to reach a diagnosis using histological examinations of specimens obtained by endoscope, which increases the medical expense. Spectroscopic analysis of bladder cancer tissues has received considerable attention due to its sensitivity to biochemical variations in the samples. The present study investigated the use of FTIR and laser Raman spectrometer as diagnose tools of bladder cancer. Fourteen bladder samples were collected from 7 patients during surgery from different hospitals without any pretreatment. FTIR, with a ceramic source, was used to differentiate between normal and cancerous bladder tissues via the change in the spectra of these samples. The investigations detected obvious spectroscopic change in the proteins (1650, 1550 cm-1), lipids (2925, 2850 cm-1) and nucleic acid (1080, 1236 cm-1). With FT Raman spectrometer supplied with Nd:YAG laser, as an excitation light source, samples were studied and significant differences between the normal and cancerous bladder tissues were found around Raman shifts of 1650 cm-1, 1440 cm-1 , 1270 cm-1 and 1080 cm-1 . The comparison between the two techniques showed that Raman spectroscopy holds much promising as a rapid, sensitive, nondestructive method, and easy to use as an alternative method for identification and diagnosis of bladder cancerous tissues.

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