Open AccessEffects of Semen Processing on Sperm Function: Differences between Swim-Up and Density Gradient Centrifugation
Author(s) -
Gabriela Hernández-Silva,
Aideé S. López-Torres,
Israel Maldonado Rosas,
Esperanza Mata-Martínez,
Fernando Larrea,
Víctor Torres-Flores,
Claudia L. Treviño,
Mayel Chirinos
Publication year - 2021
Publication title -
the world journal of men's health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.711
H-Index - 10
eISSN - 2287-4690
pISSN - 2287-4208
DOI - 10.5534/wjmh.200115
Subject(s) - hyperactivation , sperm , capacitation , tyrosine phosphorylation , semen , acrosome reaction , differential centrifugation , andrology , centrifugation , calcium , density gradient , sperm motility , biology , calcium in biology , semen analysis , microbiology and biotechnology , chemistry , intracellular , phosphorylation , medicine , infertility , biochemistry , genetics , physics , quantum mechanics , pregnancy
Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated. However, sperm function investigations results are sometimes contradictory, probably as a result of the use of different sperm processing techniques. In this work, we underwent a systematic functional comparison of human sperm samples simultaneously processed by swim-up and density gradient centrifugation, which are the preferred sperm processing methods used in basic and clinical laboratories.