Open AccessVitamin C increases the apoptosis via up-regulation p53 during cisplatin treatment in human colon cancer cells
Author(s) -
Sung-Ho An,
Jung-Hoon Kang,
Dong-Heui Kim,
Myeong-Seon Lee
Publication year - 2011
Publication title -
bmb reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.511
H-Index - 77
eISSN - 1976-670X
pISSN - 1976-6696
DOI - 10.5483/bmbrep.2011.44.3.211
Subject(s) - cisplatin , apoptosis , downregulation and upregulation , flow cytometry , cancer research , programmed cell death , colorectal cancer , dna fragmentation , cancer cell , blot , uvb induced apoptosis , cell growth , cell culture , chemistry , biology , microbiology and biotechnology , cancer , chemotherapy , caspase , biochemistry , genetics , gene
Vitamin C (VC) is an important antioxidant and enzyme co-factor that works by stimulating the immune system and protecting against infections. It is well known that melanoma cells are more susceptible to VC than any other tumor cells. However, the role of VC in the treatment of colon cancer has not been studied. Cisplatin (CDDP) is a DNA damaging agent and is widely used for treating cancer, while the role of p53 in CDDP-induced cell death has been stressed. Using cell growth assays, morphological methods, Western blotting, flow cytometry, and DNA fragmentation analysis, we measured the expression of p53 level involved in the effect of VC on CDDP-induced apoptosis of HCT116, a human colon cancer cell line. CDDP plus VC treatment resulted in significantly increased apoptosis along with upregulation of p53 compared to untreated cells and/or CDDP-treated cells. These results suggest that VC enhanced CDDP sensitivity and apoptosis via upregulation of p53.
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