Open AccessIL-18 gene expression pattern in exogenously treated AML cells
Author(s) -
Min-Ji Seo,
Minha Park,
Yeon-Joo Yook,
Young-Sook Kwon,
Young-Ju Suh,
Min-Jung Kim,
Daeho Cho,
Jong-Hoon Park
Publication year - 2008
Publication title -
bmb reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.511
H-Index - 77
eISSN - 1976-670X
pISSN - 1976-6696
DOI - 10.5483/bmbrep.2008.41.6.461
Subject(s) - gene , biology , gene expression , myeloid leukemia , cancer research , leukemia , immune system , unigene , microbiology and biotechnology , transcription factor , immunology , genetics , transcriptome
IL-18 production may enhance immune system defense against KG-1 cells ; NB4 cells, which are associated with good prognosis, do not produce IL-18. In this study, we treated KG-1 cells with IL-18 and used microarray technology to assess subsequent effects on gene expression. In UniGene-array of 7488 human genes, expression of 57 genes, including stress related genes, increased at least 2-fold, whereas expression of 48 genes decreased at least 2-fold. Following exogenous exposure of KG-1 cells to IL-18, expression of CRYGC, NF(kappa)BIA and NACA gene were monitored. The latter is a transcriptional coactivator potentiating c-Jun-mediated transcription. NF(kappa)BIA is an inhibitor of NF(kappa)B, and affects growth regulation, apoptosis and hypoxic stress. Studies, such as this one, are beginning to clarify the differences between cells associated with good and bad cancer prognoses, which may ultimately assist in medical treatment for acute myeloid leukemia.
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