Open AccessDownregulation of matrix metalloproteinases in hyperplastic dental follicles results in abnormal tooth eruption
Author(s) -
SeongGon Kim,
MyungHee Kim,
Chang-Hoon Chae,
YounKwan Jung,
JeYong Choi
Publication year - 2008
Publication title -
bmb reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.511
H-Index - 77
eISSN - 1976-670X
pISSN - 1976-6696
DOI - 10.5483/bmbrep.2008.41.4.322
Subject(s) - dental follicle , matrix metalloproteinase , downregulation and upregulation , immunohistochemistry , microbiology and biotechnology , gene expression , microarray analysis techniques , biology , alkaline phosphatase , connective tissue , real time polymerase chain reaction , microarray , gene , andrology , pathology , chemistry , medicine , immunology , biochemistry , enzyme , genetics , stem cell
In this study, we compared the gene expression profiles of non-syndromic hyperplastic dental follicle (HDF) fibroblasts and normal dental follicle (NDF) fibroblasts using cDNA microarrays, quantitative PCR, and immunohistochemical staining. Microarray analysis showed that several collagens genes were upregulated in the HDFos, including collagen types I, IV, VIII, and XI and TIMP-1, -3, and -4 (fold ratio > 2.0). In contrast, the expression of MMP-1, -3, -10, and -16 together with IL-8 was more than two fold downregulated. The differential expression of the genes encoding alkaline phosphatase, MMP-1, -3, -8, and IL-8 was confirmed by quantitative RT-PCR, while that of 24 HDFs and 18 NDFs was confirmed by immunohistochemical analysis. However, HDFs showed stronger expression of MMP-3 than NDFs (P < 0.001). Collectively, these results indicate that defective regulation of MMPs mediating connective tissue remodeling may be responsible for abnormal tooth eruption.
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