Chemical Constituen from an Endophytic Fungus Aspergillus sp (SbD5) Isolated from Sambiloto (Andrographis paniculata Nees) | Zendy

Elfita Elfita | Zendy; Munawar Munawar | Zendy; Muharni Muharni | Zendy; IHSAN IVANTRI | Zendy

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Chemical Constituen from an Endophytic Fungus Aspergillus sp (SbD5) Isolated from Sambiloto (Andrographis paniculata Nees)

Author(s) -

Elfita Elfita,

Munawar Munawar,

Muharni Muharni,

IHSAN IVANTRI

Publication year - 2015

Publication title -

microbiology indonesia

Language(s) - English

Resource type - Journals

eISSN - 2087-8575

pISSN - 1978-3477

DOI - 10.5454/mi.9.2.5

Subject(s) - bacteriocin , biology , lactobacillus plantarum , ammonium sulfate precipitation , microbiology and biotechnology , andrographis paniculata , fungus , chemistry , bacteria , size exclusion chromatography , botany , biochemistry , antimicrobial , lactic acid , medicine , genetics , alternative medicine , pathology , enzyme

Lactobacillus plantarum U10 produced bacteriocin U10 which was isolated from a traditionally fermented food “tempoyak” from Sumatera Island in Indonesia. Production of the bacteriocins started at early exponential phase and reached maximum level at early stationary phase. Furthermore, plantaricins U10 was purified by ammonium sulphate precipitation followed by gel filtration chromatography. L. plantarum U10 produced two bacteriocins with a molecular mass of approximately 4.5 and 9.8 kDa by SDS-PAGE. The mode of action of plantaricins U10 was identified as bactericidal agents against Salmonella typhi ATCC25241 as proven by CFU counting and SEM micrographs that showed differences in cell structures between treated cells and the non-treated control. SEM examination also confirmed structural destruction of membrane cells integrity and considerable morphological alteration of S.typhi.

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