Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil | Zendy

YUDHIE ISTIANTO | Zendy; Raden Setyo Adji Koesoemowidodo | Zendy; Yoshio Watanabe | Zendy; Hardaning Pranamuda | Zendy; Bambang Marwoto | Zendy

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Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil

Author(s) -

YUDHIE ISTIANTO,

Raden Setyo Adji Koesoemowidodo,

Yoshio Watanabe,

Hardaning Pranamuda,

Bambang Marwoto

Publication year - 2012

Publication title -

microbiology indonesia

Language(s) - English

Resource type - Journals

eISSN - 2087-8575

pISSN - 1978-3477

DOI - 10.5454/mi.6.1.7

Subject(s) - micromonospora , isolation (microbiology) , phenol , actinomyces , microbiology and biotechnology , agar , chemistry , biology , streptomyces , nuclear chemistry , chromatography , bacteria , organic chemistry , genetics

Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 μg/mL) and nystatin (50 μg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively

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