Horizontal Transfer of the “Popcorn-Effect” Strain of Wolbachia from Drosophila melanogaster to Stomoxys calcitrans
Author(s) -
Endang Srimurni Kusmintarsih
Publication year - 2009
Publication title -
microbiology indonesia
Language(s) - English
Resource type - Journals
eISSN - 2087-8575
pISSN - 1978-3477
DOI - 10.5454/mi.3.3.5
Subject(s) - stomoxys , wolbachia , stable fly , biology , hemolymph , drosophila melanogaster , zoology , pupa , larva , mating , instar , anatomy , botany , genetics , muscidae , host (biology) , gene
The Wolbachia containing haemolymph from Wolbachia infected Drosophila third/fourth instar larvae was transferred through microinjection into 5-days-old pupae of the blood sucking stable fly, Stomoxys calcitrans. There was no previous record of Wolbachia being present in S. calcitrans. From a total of 682 emerging adults, 236 were females and of these, seven females were tested positive (approximately 3%) for Wolbachia infection. Using electron microscopy, it was shown that Wolbachia were present in the muscle tissues of S. calcitrans.
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