Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants | Zendy

Si Won Lee | Zendy; Jin-Ho Kim | Zendy; Ji-Young Choi | Zendy; WonCheoul Jang | Zendy

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Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants

Author(s) -

Si Won Lee,

Jin-Ho Kim,

Ji-Young Choi,

WonCheoul Jang

Publication year - 2015

Publication title -

the plant pathology journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.542

H-Index - 29

eISSN - 2093-9280

pISSN - 1598-2254

DOI - 10.5423/ppj.nt.06.2015.0110

Subject(s) - loop mediated isothermal amplification , reverse transcription loop mediated isothermal amplification , quarantine , streak , plant quarantine , virology , biology , polymerase chain reaction , horticulture , reverse transcriptase , chemistry , dna , genetics , gene , ecology , mineralogy

We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.

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