Optimization of Cellulase Production from Bacteria Isolated from Soil

Cellulase-producing bacteria were isolated from soil and identified as Pseudomonas fluorescens , Bacillus subtilIs , E. coli , and Serratia marcescens . Optimization of the fermentation medium for maximum cellulase production was carried out. The culture conditions like pH, temperature, carbon sources, and nitrogen sources were optimized. The optimum conditions found for cellulase production were 40°C at pH 10 with glucose as carbon source and ammonium sulphate as nitrogen source, and coconut cake stimulates the production of cellulase. Among bacteria, Pseudomonas fluorescens is the best cellulase producer among the four followed by Bacillus subtilis , E. coli , and Serratia marscens .