Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis | Zendy

Samar Anis Al-Nahhas | Zendy; Rania M. Kaldas | Zendy

Open Access

Characterization of Leishmania Species Isolated from Cutaneous Human Samples from Central Region of Syria by RFLP Analysis

Author(s) -

Samar Anis Al-Nahhas,

Rania M. Kaldas

Publication year - 2013

Publication title -

isrn parasitology

Language(s) - English

Resource type - Journals

ISSN - 2314-4076

DOI - 10.5402/2013/308726

Subject(s) - leishmania tropica , restriction fragment length polymorphism , cutaneous leishmaniasis , leishmania , polymerase chain reaction , leishmaniasis , leishmania major , biology , lesion , virology , microbiology and biotechnology , medicine , genetics , pathology , gene , parasite hosting , world wide web , computer science

Cutaneous leishmaniasis (CL) is an endemic disease and a public health problem in Hama governorate located in the central region of Syria. The aim of this study was to characterize Leishmania species isolated from human skin samples. A polymerase chain reaction, restriction fragment length polymorphism (PCR-RFLP) assay, was performed on skin lesion material samples from 32 patients with confirmed CL by direct microscopic examination in order to prove its usefulness and efficiency for identification of Leishmania species. Leishmania tropica ( L. tropica ) is confirmed as an etiologic agent of CL in this area.

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