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The present paper is an attempt to investigate the eye irritation potential of aqueous leaf extract of  Achyranthes aspera  by  in vitro , Hen's Egg Chorioallantoic Membrane Test (HET-CAM) and  in vivo  acute eye irritation test in rabbits. The irritation score (IS) obtained after treatment of the extract on HET-CAM is 0.07 and that of in rabbits is 0.55, Which does not comes under either category 1 or 2 as per the harmonized integrated classification system. The aqueous extract of  Achyranthes aspera  showed no eye irritation properties both  in vitro  and  in vivo  methods when compared with negative control whereas positive controls showed eye irritation potential.

Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method