Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications | Zendy

Ajeetkumar Patil | Zendy; K.S. Choudhari | Zendy; V.K. Prabhu | Zendy; V. K. Unnikrishnan | Zendy; Sujatha Bhat | Zendy; Keerthilatha M. Pai | Zendy; V. B. Kartha | Zendy; Santhosh Chidangil | Zendy

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Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications

Author(s) -

Ajeetkumar Patil,

K.S. Choudhari,

V.K. Prabhu,

V. K. Unnikrishnan,

Sujatha Bhat,

Keerthilatha M. Pai,

V. B. Kartha,

Santhosh Chidangil

Publication year - 2012

Publication title -

isrn spectroscopy

Language(s) - English

Resource type - Journals

ISSN - 2090-8776

DOI - 10.5402/2012/643979

Subject(s) - proteomics , chromatography , high performance liquid chromatography , fluorescence , chemistry , laser induced fluorescence , biochemistry , optics , physics , gene

This paper describes the sensitivity study and performance evaluation of high-performance liquid chromatography-laser-induced fluorescence detection (HPLC-LIF) system assembled in our laboratory for proteomics applications. The limits of Detection (LOD) of several serum proteins have been estimated with this instrument and are found to be much lower compared to other commonly used proteomics techniques like SELDI, MALDI, 2-D-SDS-PAGE, and so forth. Techniques for improving the LOD still further with similar setup are briefly discussed. Using the system, protein profiles of serum in normal, malignant, and premalignant conditions were recorded for different malignancy situations.

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