Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro | Zendy

Christian Jreyssaty | Zendy; Qin Shi | Zendy; Huijie Wang | Zendy; XingPing Qiu | Zendy; Françoise M. Winnik | Zendy; Xiaoling Zhang | Zendy; Kerong Dai | Zendy; Mohamed Benderdour | Zendy; Julio Fernandes | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro

Author(s) -

Christian Jreyssaty,

Qin Shi,

Huijie Wang,

XingPing Qiu,

Françoise M. Winnik,

Xiaoling Zhang,

Kerong Dai,

Mohamed Benderdour,

Julio Fernandes

Publication year - 2012

Publication title -

isrn pharmaceutics

Language(s) - English

Resource type - Journals

eISSN - 2090-6153

pISSN - 2090-6145

DOI - 10.5402/2012/369270

Subject(s) - transfection , chitosan , in vitro , immunogenicity , dna , microbiology and biotechnology , chemistry , genetic enhancement , intracellular , cell culture , gene delivery , biophysics , biochemistry , gene , biology , antigen , immunology , genetics

Nonviral cationic polymers like chitosan can be combined with DNA to protect it from degradation. The chitosan is a biocompatible, biodegradable, nontoxic, and cheap polycationic polymer with low immunogenicity. The objective of this study was to synthesize and then assess different chitosan-DNA nanoparticles and to select the best ones for selective in vitro transfection in human epidermoid carcinoma (KB) cell lines. It revealed that different combinations of molecular weight, the presence or absence of folic acid ligand, and different plasmid DNA sizes can lead to nanoparticles with various diameters and diverse transfection efficiencies. The intracellular trafficking, nuclear uptake, and localization are also studied by confocal microscopy, which confirmed that DNA was delivered to cell nuclei to be expressed.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research