Development and Evaluation of Environmentally Benign Aqueous Two Phase Systems for the Recovery of Proteins from Tannery Waste Water | Zendy

Raja Selvaraj | Zendy; V. Ramachandra Murty | Zendy

Open Access

Development and Evaluation of Environmentally Benign Aqueous Two Phase Systems for the Recovery of Proteins from Tannery Waste Water

Author(s) -

Raja Selvaraj,

V. Ramachandra Murty

Publication year - 2012

Publication title -

isrn chemical engineering

Language(s) - English

Resource type - Journals

ISSN - 2090-861X

DOI - 10.5402/2012/290471

Subject(s) - sodium citrate , aqueous solution , chemistry , sodium , polyethylene glycol , partition coefficient , peg ratio , tetrahydrate , salting out , chromatography , biochemistry , organic chemistry , crystal structure , medicine , finance , pathology , economics

Aqueous two phase systems (ATPSs) containing high molecular weight polyethylene glycol, PEG (10000), and completely biodegradable citrate salts (sodium citrate, potassium citrate, and ammonium citrate) with water were developed to recover the waste water proteins from a model tannery waste water system. The variations in the phase diagram were explained on the basis of ionic radius of cations, Gibbs free energy of hydration () of cations, and effective excluded volume (EEV) of salts. The salting-out capability of the cations followed the sequence: Sodium citrate > Potassium citrate > Ammonium citrate. Setschenow-type equations were used to correlate tie-line compositions. During partitioning studies, recovery of tannery waste water proteins in PEG 10000

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