Determination of Aflatoxin M1 in Milk by ELISA Technique in Mashad (Northeast of Iran) | Zendy

Ali Mohamadi Sani | Zendy; Mohamad Khezri | Zendy; Halime Moradnia | Zendy

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Determination of Aflatoxin M1 in Milk by ELISA Technique in Mashad (Northeast of Iran)

Author(s) -

Ali Mohamadi Sani,

Mohamad Khezri,

Halime Moradnia

Publication year - 2012

Publication title -

isrn toxicology

Language(s) - English

Resource type - Journals

eISSN - 2090-6196

pISSN - 2090-6188

DOI - 10.5402/2012/121926

Subject(s) - aflatoxin , pasteurization , contamination , mathematics , algorithm , detection limit , european commission , food science , european union , chemistry , statistics , biology , ecology , business , economic policy

The aim of this study was to detect the amount of aflatoxin M 1 (AFM 1 ) in pasteurized milk samples in Mashad in northeast of Iran. For this purpose, 42 milk samples were collected from retail stores during fall 2011 and analyzed for AFM 1 by enzyme-linked immunosorbent assay (ELISA) technique. All the analyses were done twice. Results showed presence of AFM 1 in 97.6% of the examined milk samples by average concentration of 23 ± 16 ppt and contamination level ranging between 6 and 71 ppt. The concentration of AFM 1 in all the samples was lower than the Iranian national standard and Food and Drug Administration limits (500 ppt), and, only in 3 (1.6%) samples, AFM 1 concentration was more than the maximum tolerance limit (50 ppt) accepted by European Union and Codex Alimentarius Commission. According to our findings and previous studies, AFM 1 contamination of milk is not a concern in this region, and the regional standard of AFM 1 contamination in milk might be changed to lower than 100 ppt.

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