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Immunorecognition of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Some Normal and Malignant Tissues from Genitourinary System
Author(s) -
Rancés Blanco,
Mercedes Cedeño,
Xiomara Escobar,
Damián Blanco,
Charles E. Rengifo,
Milagros Frómeta,
Rosa Irene Alvarez,
Enrique Rengifo,
Adriana Carr
Publication year - 2011
Publication title -
isrn pathology
Language(s) - English
Resource type - Journals
eISSN - 2090-570X
pISSN - 2090-5718
DOI - 10.5402/2011/953803
Subject(s) - immunohistochemistry , genitourinary system , pathology , ganglioside , antigen , glycolipid , kidney , monoclonal antibody , antibody , biology , prostate , ovary , cancer , medicine , immunology , endocrinology , anatomy , biochemistry
N-glycolyl neuraminic acid has been considered as a tumour-associated antigen forming both glycolipid and glycoprotein, expressed in some human malignant cells. In this work, we evaluate the 14F7 Mab (an IgG1 murine highly specific to N-glycolyl GM3 ganglioside) reactivity in a variety of genitourinary-system-derived tumors as well as in their normal counterparts. Immunohistochemical assays with 14F7 followed by anti-mouse biotinylated antibody and ABC/HRP system using formalin-fixed and paraffin-embedded tissues were carried out. In normal tissues, 14F7 was reactive only in renal tubules of kidney (2/6) and in the stromal component and blood vessels of ovary (3/5). Tumors of kidney (12/38), urinary bladder (8/9), breast (41/42), ovary (21/34), testis (4/5), prostate (17/20), and uterus (5/14) as well as prostatic nodular hyperplasia (5/8) were stained with 14F7. N-glycolyl GM3 recognized by 14F7 could be considered as one attractive target for both active and passive immunotherapy of genitourinary malignancies expressing this molecule.

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