Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds | Zendy

Monia Blibech | Zendy; Raoudha Ellouz Ghorbel | Zendy; Fatma Châari | Zendy; Ilyes Dammak | Zendy; Fatma Bhiri | Zendy; Mohamed Neifar | Zendy; Semia Ellouz Châabouni | Zendy

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Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds

Author(s) -

Monia Blibech,

Raoudha Ellouz Ghorbel,

Fatma Châari,

Ilyes Dammak,

Fatma Bhiri,

Mohamed Neifar,

Semia Ellouz Châabouni

Publication year - 2011

Publication title -

isrn microbiology

Language(s) - English

Resource type - Journals

eISSN - 2090-7486

pISSN - 2090-7478

DOI - 10.5402/2011/938347

Subject(s) - fermentation , acacia , substrate (aquarium) , food science , penicillium , inducer , biology , chemistry , botany , biochemistry , ecology , gene

By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.

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