Bactericidal Effects of Diode Laser Irradiation on Enterococcus faecalis Using Periapical Lesion Defect Model | Zendy

Masato Nagayoshi | Zendy; Tatsuji Nishihara | Zendy; Keisuke Nakashima | Zendy; Shigetsugu Iwaki | Zendy; Ker-Kong Chen | Zendy; Masamichi Terashita | Zendy; Chiaki Kitamura | Zendy

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Bactericidal Effects of Diode Laser Irradiation on Enterococcus faecalis Using Periapical Lesion Defect Model

Author(s) -

Masato Nagayoshi,

Tatsuji Nishihara,

Keisuke Nakashima,

Shigetsugu Iwaki,

Ker-Kong Chen,

Masamichi Terashita,

Chiaki Kitamura

Publication year - 2011

Publication title -

isrn dentistry

Language(s) - English

Resource type - Journals

eISSN - 2090-438X

pISSN - 2090-4371

DOI - 10.5402/2011/870364

Subject(s) - enterococcus faecalis , irradiation , laser , microbiology and biotechnology , lesion , diode , dentistry , materials science , chemistry , optoelectronics , medicine , pathology , biology , optics , escherichia coli , physics , biochemistry , nuclear physics , gene

Objective . Photodynamic therapy has been expanded for use in endodontic treatment. The aim of this study was to investigate the antimicrobial effects of diode laser irradiation on endodontic pathogens in periapical lesions using an in vitro apical lesion model. Study Design . Enterococcus faecalis in 0.5% semisolid agar with a photosensitizer was injected into apical lesion area of in vitro apical lesion model. The direct effects of irradiation with a diode laser as well as heat produced by irradiation on the viability of microorganisms in the lesions were analyzed. Results . The viability of E. faecalis was significantly reduced by the combination of a photosensitizer and laser irradiation. The temperature caused by irradiation rose, however, there were no cytotoxic effects of heat on the viability of E. faecalis . Conclusion . Our results suggest that utilization of a diode laser in combination with a photosensitizer may be useful for clinical treatment of periapical lesions.

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