Open AccessMODULATORY ANTI- DIARRHEAL EFFECTS OF ASCORBIC ACID IN SWISS ALBINO MICE
Author(s) -
Mahmuda Akter Mukta,
Rajib Hossain,
Farhana Faria,
Salma Islam,
Chandan Kumar Sarkar,
Abul Hasan Muhammad Bashar,
Ripon Khalipha,
Olubunmi Atolanı,
Muhammad Torequl Islam
Publication year - 2021
Publication title -
khulna university studies
Language(s) - English
Resource type - Journals
eISSN - 2789-2697
pISSN - 1563-0897
DOI - 10.53808/kus.2021.18.01.2103-l
Subject(s) - loperamide , chemistry , propranolol , ascorbic acid , diarrhea , nifedipine , prazosin , pharmacology , antagonist , medicine , receptor , biochemistry , food science , calcium , organic chemistry
Ascorbic acid (AA) has been reported for the management of diarrhea. The anti-diarrheal potential and modulatory activities of AA on some commonly used anti-diarrheal drugs were investigated. For this purpose, the activities of AA on castor oil-induced diarrhea in Swiss mice were examined. As standard anti-diarrheal agents, we used prazosin, propranolol, loperamide, and nifedipine with or without AA. The results revealed that AA at 25 mg/kg (i.p.) and all other standard drugs exhibited significant (p < 0.05) diarrheal attenuating activities in mice. However, the impact was more pronounced in the loperamide and propranolol groups. AA administrated with prazosin and propranolol had a higher rate of latent periods and a lower rate of diarrheic secretion during the study period (4 h) than that of the other single or mixed groups. Furthermore, a molecular docking study illustrated that AA displayed good binding affinities with (α1) (–5.2 Kcal/mol), α2b (–5.4 Kcal/mol), α2c (-5.6 Kcal/mol), β1(–5.3 Kcal/mol) and β2(–6.4 Kcal/mol) adrenoceptors. Of note, AA exerted a significant anti-diarrheal effect and it was seen to modulate the anti-diarrheal effects of α- and β-adrenergic receptor blocking agents in Swiss mice.