DESINFESTAÇÃO E MEIO DE CULTURA PARA O ESTABELECIMENTO In Vitro DE SEGMENTOS NODAIS DE Liquidambar styraciflua

Disinfestation and culture medium for the in vitro establishment of Liquidambar styraciflua nodal segments . The objective of this research was to test the cu lture medium sterilization for the in vitro establishment of Liquidambar styraciflua L. nodal segments. Ministumps, from which the explants were collected, were propagated by cutting process and managed in clonal mini garden under semihydroponic system in a sand bed. The experiment was conducted in a completely randomized design under factorial arrangement (3x2x2); the factors we re: three clones (L 26, L 35 and L 63), two sterilization methods (A1 - sodium hypochlorite (2. 5% v/v of active chlorine) during 10 minutes; A2 explants immersed during 40 minutes in a solution o f benomyl 1% w/v) and two culture mediums (WPM and MS), with four replications. The clones did not differ in relation to the asepsis and culture medium. In average, 4% of the explants were free of contamination. However, almost 80% of the material presented bacterial contamination, what in dicated the necessity of developing a more efficient sterilization protocol. Although only a l ittle difference between the culture mediums had been observed, the MS medium showed superiority in relation to the WPM regarding the majority of the morphological characteristics studied.