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O metodo padrao de Kjeldahl vem sendo substituido pelo metodo de combustao Dumas na determinacao do nitrogenio total (NT) em alimentos. Estes procedimentos foram utilizados para avaliar amostras de rotina em laboratorios de nutricao animal (concentrados, forragens, excreta e conteudo duodenal). A covariavel nitrato (N-NO3) interferiu na determinacao do NT em funcao da metodologia empregada. Foi observada uma correlacao positiva entre os metodos utilizados para cada grupo avaliado: ingredientes volumosos, Kjeldahl-N = 1.1661 + 0.9216 Dumas-N (R2=0.99); alimentos energeticos, Kjeldahl-N = 0.6376 + 0.9445 Dumas-N (R2=0.72) e alimentos proteicos, Kjeldahl- N = 6.5638 + 0.8615 Dumas-N (R2=0.97). As medias para NT dos alimentos, ajustadas para a covariavel, nao diferiram entre os metodos (P > 0,05). O desvio padrao do teor de N dentro e entre os metodos utilizados estao dentro dos intervalos permitidos pela literatura, o que, associado ao fato da metodologia de Dumas consistir em uma tecnica nao poluente ao ambiente, indica ser esta metodologia capaz de substituir com vantagens o procedimento de Kjeldahl em analises de rotina em laboratorios de nutricao animal. Abstract The Kjeldahl procedure has been replaced by Dumas combustion procedure for total N determination in feeds. These methods were compared using routine samples in the animal nutrition laboratory (concentrates, forages, excreta and duodenal content). NO3-N covariate interfered on total N determination according to the procedure used. A significant correlation for crude protein (CP) content was observed between methods used for each group: grass silage component, Kjeldahl-N = 1.1661 + 0.9216 Dumas-N (R2=0.99); energy feeds, Kjeldahl-N = 0.6376 + 0.9445 Dumas-N (R2=0.72) and protein feeds, Kjeldahl-N = 6.5638 + 0.8615 Dumas-N (R2=0.97). Means for total N contents from components, adjusted by covariance, were not different (P > 0.05) between both methods. Considering that the standard deviation for N content obtained by the two methods stayed within the intervals observed in the literature, corroborated by the fact that Dumas combustion procedure using the analyzer LECOFP 528 LC is not harmful to the environment it was concluded, that this method can replace with advantages the Kjeldahl procedure in routine animal nutrition N analyzes.

APPLICATION OF KJELDAHL AND DUMAS COMBUSTION METHODS FOR NITROGEN ANALYSIS