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Celulas hepaticas de Metynnis roosevelti obtidas de cultivo celular primario foram submetidas a ensaios de citotoxicidade in vitro com os principios ativos 2,4-D+MCPA (acido 2,4 diclorofenoxiacetico+acido 4-cloro-2-metilfenoxiacetico). Para determinacao da CL50 foram utilizados dois metodos: um por meio da viabilidade celular com vermelho neutro e outro atraves da observacao direta das celulas afetadas com microscopio invertido IM. A CL50 media das celuas hepaticas de Metynnis roosevelti em cultivo primario expostas ao 2,4D+MCPA por 24 horas, foi estabelecida entre 0,0265 g/ml e 0,0312 g/ml. Abstract In vitro citotoxicity of 2,4-D + MCPA (2,4-dichlorophenoxyacetic acid + 4-chloro-2- methylphenoxyacetic acid) tests were carried out with primary cultures of hepatic cells from Metynnis roosevelti (Pisces, Teleostei, Characidae). To establish the CL50 values, two different methods were used: the measurement of cell viability by means of neutral red and by the direct microscopic observation of the defective cells by means of an IM inverted microscope. The average CL50 value of the hepatic cells in primary cultures exposed to the chlorophenoxyacetates used, has been established between 0.0265 and 0.0312 g/ml.

CITOTOXICIDADE IN VITRO DE CLOROFENOXIACETATO COM CÉLULAS HEPÁTICAS DE Metynnis roosevelti (PISCES, CHARACIDAE) EM CULTIVO

CITOTOXICIDADE IN VITRO DE CLOROFENOXIACETATO COM CÉLULAS HEPÁTICAS DE Metynnis roosevelti (PISCES, CHARACIDAE) EM CULTIVO