OCCURRENCE OF CRYPTOSPORIDIUM SP. IN DOGS AND CATS FROM CURITIBA AND ITS METROPOLITAN AREA | Zendy

Martha Grecca | Zendy; Vanete ThomazSoccol | Zendy; Magda C Costa Ribeiro | Zendy; Jessé Henrique Truppel | Zendy; Juliana Tracz Pereira | Zendy; Sílvia Cristina Osaki | Zendy

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OCCURRENCE OF CRYPTOSPORIDIUM SP. IN DOGS AND CATS FROM CURITIBA AND ITS METROPOLITAN AREA

Author(s) -

Martha Grecca,

Vanete ThomazSoccol,

Magda C Costa Ribeiro,

Jessé Henrique Truppel,

Juliana Tracz Pereira,

Sílvia Cristina Osaki

Publication year - 2013

Publication title -

archives of veterinary science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.15

H-Index - 9

eISSN - 2317-6822

pISSN - 1517-784X

DOI - 10.5380/avs.v18i3.29522

Subject(s) - curitiba , cats , feces , cryptosporidium , veterinary medicine , nested polymerase chain reaction , biology , polymerase chain reaction , microbiology and biotechnology , medicine , gene , philosophy , biochemistry , humanities

The present study was carried out with the aim of assess the occurrence of Cryptosporidium sp. infection in dogs and cats in Curitiba and its metropolitan area, state of Parana, Brazil. Techniques employed to detect the protozoan in fecal samples were: staining by Ziehl- Neelsen for oocysts search and nested polymerase chain reaction (nPCR) targeting the 18SSU rDNA gene. To attempt the proposed aim, 91 feces samples of dogs and 25 of cats were collected and analyzed. Ziehl-Neelsen technique was unable to detect any oocyst in both groups analyzed, showing a very low sensitivity. Results of nPCR showed an infection rate of 13.2% (12/91) and 4% (1/25) in dogs and cats respectively. The implications of these epidemiological data are discussed in this work.

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