Open AccessCHARACTERIZATION OF CANINE LEISHMANIASIS BY PCR-RFLP IN CUIABA, MATO GROSSO, BRAZIL
Author(s) -
Arleana do Bom Parto Ferreira de Almeida,
Daphine Ariadne Jesus de Paula,
Valéria Dutra,
Édson Moleta Colodel,
Luciano Nakazato,
Valéria Régia Franco Sousa
Publication year - 2012
Publication title -
archives of veterinary science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.15
H-Index - 9
eISSN - 2317-6822
pISSN - 1517-784X
DOI - 10.5380/avs.v17i2.21092
Subject(s) - leishmania chagasi , veterinary medicine , restriction fragment length polymorphism , visceral leishmaniasis , leishmania , biology , leishmaniasis , indirect immunofluorescence , virology , zoology , polymerase chain reaction , medicine , immunology , parasite hosting , antibody , genetics , gene , world wide web , computer science
Leishmaniases are neglected zoonoses that are increasing in Brazil. The dog is considered the main reservoir of the visceral form in urban areas of Brazil and also important in maintaining the cycle of transmission of the cutaneous form in endemic areas. We used PCR-RFLP to identify the species of Leishmania involved in canine infection in Cuiaba City, Mato Grosso. Samples of bone marrow and lymph were collected from 181 dogs, of which 7.2% tested positive with indirect immunofluorescence and 24.9% using PCR-RFLP; a significant difference (p ≤ 0.05), had been possible to characterize the species Leishmania (L.) chagasi . This will aid in developing prevention measures and in the control of disease in Cuiaba and the surrounding area.
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