EVALUATION OF THE INTERFERENCE OF EDTA IN THE BLOOD COUNT RELATED TO DIFFERENT CONCENTRATIONS BLOOD/ANTICOAGULANT

The hematological counters are more sophisticated now-a-days, so the demanding of the pre-analytical phase must be greater. one of the main various pre-analytical phase which interferes in the blood count results is the relationship between blood and anticoagulant. The literature reports a signicant alterations in the blood counts results when this relationship is alterated. The aim of this study was to evaluate if there is this alteration and how signicant it can be. A peripheral blood samples from 50 patients, were attended in the Clinical Laboratory of the Evangelical Hospital of Curitiba - PR were analysed. From each patient was collected 4 bottles, in tripotassium EDTA, containing 1, 2, 3 and 4 mL of blood, in the last bottle the relationship between blood and anticoagulant was corrected. In the remainnings was anticoagulant in excees. The time between the blood sample collection and the analysis of blood did not exceed 30 minutes. Statistical analysis showed no signicant difference between the four samples, besides the different concentration between blood and anticoagulant, when Cell Dyn Ruby hematological counter was used.