Desarrollo y validación de un método para la cuantificación de atorvastatina en tabletas mediante HPLC-DAD

A method for the identification and determination of Atorvastatine (ATO) in tablets by high performance liquid chromatography with diode array detector (HPLC-DAD) is proposed. Better separation of the analyte was optimized with a gradient isocratic separation by using a mobile phase composed of water adjusted to pH 3.0 with orto phosphoric acid and acetonitrile (50:50). The separation of the analyte was achieved using a C18 column 150 X 4 mm with a particle size of 5microns at a temperature of 40°C and a 245nm detector wavelength. The method was validated following the recommendations made by the International Conference of Harmonization (ICH), showing good linear relationships for the analyte (r greater than 0.998). The precision and accuracy of the results intra and inter-day was between 98.0 and 102%. The proposed method was applied successfully to the determination of the compound in pharmaceutical preparations of tablets with good accuracy and precision (without interference excipients), with which the method can be applied to routine analysis based compound. Revista Portal de la Ciencia, No. 9, diciembre 2015, pp.27-41