VScope — Data Acquisition and Analysis for Voltage-sensitive Dye Imaging Using Multiple Cameras and Electrophysiology

VScope is a software package for the acquisition and analysis of data from multiple cameras as well as electrophysiology. Its main intended purpose is to record fluorescent traces from neurons loaded with voltage-sensitive dyes along with associated electrophysiology. VScope can record simultaneously from any number of cameras and frame rates can be set independently for each camera. VScope can also record synchronized electrophysiology traces and digital channels. VScope is mainly intended to acquire fixed-duration trials, but it can also acquire electrophysiology continuously. A variety of electrical stimulation protocols can be created through an easy-to-use GUI. Funding statement: VScope was developed in conjunction with research funded by the Broad Foundations (Broad Fellowship program at Caltech); the Burroughs Wellcome Fund (Career Award at the Scientific Interface #1007977) and the National Institutes of Health (R01-NS-094403)