Bacterial isolates from burn wound infection and their antibiotic sensitivity test profile

Background: The aim of this study was to determine the antibacterial resistant pattern of aerobic bacteria isolated from burn wound infected cases admitted in burn unit at Kanti Children’s Hospital, Kathmandu, Nepal during Nov 2017-Nov 2018. Methods: In total 200 samples were collected, and that comprised 100 burns wound swabs and 100 biopsies of same patients. All samples were cultured on nutrient agar, MacConkey agar and blood agar at 37°C for 24 hours. The isolates were identified by culture, sensitivity and biochemical tests including oxidase, catalase, lactose and maltose fermentation. Their antibiotic sensitivity test was determined using Kirby Bauer disc diffusion technique. Results: The most common isolate was Pseudomonas aeruginosa (55%), followed by Staphylococcus aureus (19.29%), Klebsiella spp (11.43%), Acinetobacter spp (7.14%), Proteus spp (4.29%) and Escherichia coli (2.85%). Resistance observed in oxacillin (40%) and erythromycin (4%) to Staphylococcus aureus. Whereas all strains were susceptible to vancomycin. It was analyzed that ciprofloxacin (55.5%) & amikacin (54.0%) were found to be most effective antimicrobial agent. Other Gram negative organism were highly resistant to cefotaxime (66.0%) followed by gentamycin (60.0%). Imipenem was found to be less resistant (26%) against Pseudomonas.Conclusion: Vancomycin is the drug of choice for burn wound infection.