Comparison of Picogreen and Sytox Orange Stains in Quantitative Analysis of Extracellular Trap Formed against Toxoplasma gondii in Polymorphonuclear Leukocytes from Sheep | Zendy

Percem Atan | Zendy; Kader Yıldız | Zendy

Open Access

Comparison of Picogreen and Sytox Orange Stains in Quantitative Analysis of Extracellular Trap Formed against Toxoplasma gondii in Polymorphonuclear Leukocytes from Sheep

Author(s) -

Percem Atan,

Kader Yıldız

Publication year - 2018

Publication title -

turkish journal of parasitology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.207

H-Index - 18

eISSN - 2146-3077

pISSN - 1300-6320

DOI - 10.5152/tpd.2018.5952

Subject(s) - toxoplasma gondii , neutrophil extracellular traps , extracellular , trap (plumbing) , orange (colour) , biology , chemistry , immunology , microbiology and biotechnology , antibody , inflammation , food science , environmental engineering , engineering

The present study aimed to compare the effectiveness of two extracellular DNA dyes (Sytox Orange and PicoGreen) in quantitative measurement of the DNA that forms the backbone of the extracellular trap structures in vitro. Toward this aim, the co-culture of polymorphonuclear leucocytes (PMNs) and Toxoplasma gondii tachyzoites isolated from sheep was selected as model.

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