Cryopreservation of Toxoplasma gondii Tachyzoites and Tissue Cysts | Zendy

Mert Döşkaya | Zendy; Ayşe Caner | Zendy; Hüseyin Can | Zendy; Sultan Gülçe İz | Zendy; Aysu Değirmenci | Zendy; Adnan Yüksel Gürüz | Zendy

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Cryopreservation of Toxoplasma gondii Tachyzoites and Tissue Cysts

Author(s) -

Mert Döşkaya,

Ayşe Caner,

Hüseyin Can,

Sultan Gülçe İz,

Aysu Değirmenci,

Adnan Yüksel Gürüz

Publication year - 2013

Publication title -

turkish journal of parasitology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.207

H-Index - 18

eISSN - 2146-3077

pISSN - 1300-6320

DOI - 10.5152/tpd.2013.11

Subject(s) - cryopreservation , toxoplasma gondii , biology , toxoplasmosis , defrosting , immunology , embryo , microbiology and biotechnology , antibody , physics , air conditioning , thermodynamics

Toxoplasma gondii tachyzoites and tissue cysts are largely used for developing diagnostic assays, vaccines and in drug research as well as biochemical and molecular structure studies. Continuous passaging of tachyzoites or tissue cysts in animal models encounter ethical and economical problems and it is a time consuming procedure. Cryopreservation of tachyzoites and tissue cysts and revitalization of cryopreserved samples whenever needed, can decrease the economical loss, ethical problems and labour. In the present article, production of tachyzoites and tissue cysts in mice, preparation of samples for cryopreservation, cryopreservation of tachyzoites and tissue cysts, defrosting of cryopreserved samples and reinoculation to mice have been described in detail.

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