SÍNTESE DE PARTÍCULAS POLIMÉRICAS DE ESTIRENO/EGDMA COM PROPRIEDADES MAGNÉTICAS PARA UTILIZAÇÃO COMO MATRIZ DE IMOBILIZAÇÃO DE ENZIMAS

The objective of this work was to synthesize and characterize a magnetic copolymer obtained by suspension polymerization, aiming its application as a support for immobilizing enzymes. The influence of some factors on the polymerization yield of styrene/ triethylene glycol dimethacrylate/ magnetite (STY-TEGDMA-M) particles was studied using Taguchi's exploratory methodology. The best conditions were found at maximum levels for all the studied factors, being the resulted copolymer characterized. The morphological characteristics and incorporation of the magnetite in the polymer matrix were confirmed by the SEM, MOC, EDX, ICP-OES, FT-IR, Raman and Hysteresis analyzes. The copolymer is thermally resistant (decomposition temperature around 366 °C), showing a glass transition temperature of 84.63 °C, by the TG and DSC analyzes, respectively. The biocatalyst obtained by the immobilization of Candida rugosa lipase showed hydrolytic activity of 1949.56 ± 44.23 U.g-1, attested the suitability of the synthesized support.