ONE-STEP PURIFICATION OF C-PHYCOCYANIN EXTRACTED FROM THE WET BIOMASS OF Spirulina platensis LEB-52 | Zendy

Marina Campos Assumpção de Amarante | Zendy; L. C. Correa | Zendy; Luisa Sala | Zendy; Susana Juliano Kalil | Zendy

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ONE-STEP PURIFICATION OF C-PHYCOCYANIN EXTRACTED FROM THE WET BIOMASS OF Spirulina platensis LEB-52

Author(s) -

Marina Campos Assumpção de Amarante,

L. C. Correa,

Luisa Sala,

Susana Juliano Kalil

Publication year - 2018

Publication title -

blucher chemical engineering proceedings

Language(s) - English

Resource type - Conference proceedings

DOI - 10.5151/cobeq2018-pt.0585

Subject(s) - phycobiliprotein , spirulina (dietary supplement) , phycocyanin , chromatography , gradient elution , chemistry , elution , biomass (ecology) , cyanobacteria , high performance liquid chromatography , biology , bacteria , raw material , genetics , organic chemistry , agronomy

The aim of this work was to purify C-phycocyanin (C-PC) extracted from the wet biomass of Spirulina platensis LEB-52 by ion-exchange chromatography (IEC) with elution by pH gradient and compare it with elution by step followed by NaCl gradient, which is an optimized condition for C-PC purification. The best results were obtained when pH gradient was used, resulting in C-PC with purity of 4 and 60 % of recovery in one purification step. IEC with elution by pH gradient is rarely applied in phycobiliproteins’ purification, but the results showed that it is a promising technique for C-PC purification.

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