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The aim of this work was to purify C-phycocyanin (C-PC) extracted from the wet biomass of Spirulina platensis LEB-52 by ion-exchange chromatography (IEC) with elution by pH gradient and compare it with elution by step followed by NaCl gradient, which is an optimized condition for C-PC purification. The best results were obtained when pH gradient was used, resulting in C-PC with purity of 4 and 60 % of recovery in one purification step. IEC with elution by pH gradient is rarely applied in phycobiliproteins’ purification, but the results showed that it is a promising technique for C-PC purification.

ONE-STEP PURIFICATION OF C-PHYCOCYANIN EXTRACTED FROM THE WET BIOMASS OF Spirulina platensis LEB-52