Assessment of Efflux Activity Using H33342 Accumulation in Tigecycline-ResistantAcinetobacter baumanniiClinical Isolates | Zendy

ChoonMee Kim | Zendy; In Sun Choi | Zendy; Sook Jin Jang | Zendy; Na Ra Yun | Zendy; DongMin Kim | Zendy; Dong-Hoon Lim | Zendy; YoungJoon Ahn | Zendy; Seong Ho Kang | Zendy; Geon Park | Zendy; Dae Soo Moon | Zendy

Open Access

Assessment of Efflux Activity Using H33342 Accumulation in Tigecycline-ResistantAcinetobacter baumanniiClinical Isolates

Author(s) -

ChoonMee Kim,

In Sun Choi,

Sook Jin Jang,

Na Ra Yun,

DongMin Kim,

Dong-Hoon Lim,

YoungJoon Ahn,

Seong Ho Kang,

Geon Park,

Dae Soo Moon

Publication year - 2017

Publication title -

annals of clinical microbiology

Language(s) - English

Resource type - Journals

eISSN - 2288-6850

pISSN - 2288-0585

DOI - 10.5145/acm.2017.20.4.90

Subject(s) - tigecycline , efflux , acinetobacter baumannii , microbiology and biotechnology , biology , antibiotics , bacteria , biochemistry , genetics , pseudomonas aeruginosa

Background: Tigecycline resistance has emerged recently and has shown diverse mechanisms. The aim of this study was to assess the role of efflux activity in tigecycline resistance in 120 clinical isolates of A. baumannii using two methods: the H33342 accumulation assay and adeB real-time reverse transcriptase polymerase chain reaction. In addition, we analyzed the correlation between the expression level of adeB and H33342 accumulation level. Methods: A. baumannii clinical isolates was divided into tigecycline-resistant (49 strains), intermediate (40 strains), and susceptible (31 strains) groups. The H33342 accumulation was measured in the absence or presence of the efflux pump inhibitor carbonyl cyanide 3-chlorophenylhydrazone (CCCP). Real-time RTPCR was performed to determine the relative expression of the adeB gene in A. baumannii clinical isolates. Results: The level of H33342 accumulation in the resistant group was relatively lower than those in the other groups. The addition of CCCP caused a significantly increased fold change in H33342 accumulation in the tigecycline-resistant group. Significant difference in the fold change level in H33342 accumulation was found between tigecycline-susceptible and resistant isolates. Those findings support the role of efflux pumps of which substrates are H33342 in the resistance of tigecycline. Significant differences in the relative expression levels of adeB were shown between tigecycline-susceptible and resistant groups also. Conclusion: The results showed that several efflux pumps of which substrates were H33342 can contribute to tigecycline resistance. The adeB overexpression can also contribute to tigecycline resistance. It is possible that efflux pumps other than adeB efflux pumps contribute to tigecycline resistance because there was no correlation between fold change level in H33342 accumulation and adeB expression level. (Ann Clin Microbiol 2017;20:90-96)

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