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The effects of chromium bioaccumulation in Pseudokirchneriella subcapitata were evaluated by Central Composite Design (CCD), factorial 2 2 and Response Surface Methodology (RSM). All the models of regression generated by CCD were highly significant, with R 2 between 77 and 88%, which is the percentual variability in the response that the model can account for. This is indicative of a satisfactory representation of the process models whose data can be used for simulations of response. The maximum shrinkage biovolume presented 28–69% reduction compared to controls. Results from this study suggest that the smaller algal cells amplify metal binding sites, leading to an increased bioaccumulation and a consequential increased capacity to accumulate chromium. Nevertheless, the absorption capacity decreases for more elevated chromium concentrations and for longer exposure. Keywords : Algae, Biovolume, Central Composite Design, Metal, Selenastrum capricornutum .

journal of the brazilian society of ecotoxicology

Assessment of chromium bioaccumulation in Pseudokirchneriella subcapitata (Korshikov) Hindak by the Central Composite Design (CCD) and Response Surface Methodology (RSM)